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WET LAB - MAJOR EQUIPMENTS

S. No. Equipment Configuration and Application
1.
HPLC System
AKTA PURIFIER 10
High Performance Liquid Chromatography system.
Different types of ion exchange columns are available for eluting the protein sample. HiTrap - CM Sepharose (5ml), HiTrap - CM Sepharose (5ml), DEAE sepharose (30ml), Size Exclusion column, etc., are few examples among the columns we have. HPLC is connected with the dedicated PC for automated control.

2.

UV-Visible Spectrophotometer
Shimadzu Model - Uv-1800
Eight different standard operational modes are available to perform these studies. Microprocessor based UV-VIS Spectrophotometer with high resolution LCD display and soft keypad for operation on 220V/50 Hz with glass and quartz cuvette. It is connected with desktop system to enable complete PC control. Various biomolecule samples can be estimated accurately with a Wide wavelength range of 1,100 nm to 190 nm, built in validation program, diagnostic and security functions.

3.

ELISA Reader
Versamax Model 2020
It is provided with a 96 wells microplate with a wavelength range of 400-750 mm. the measuring range for the instrument is 0-4.000 Abs and the measuring time is about 3 seconds per plate in a continuous mode. It supports the absorbance, dual wavelength and multi wavelength methods.

4.

Gel Documentation System
Syngene GBOX/EF
Has a UV-to-white light converter for viewing protein gels. It has a microprocessor based controller with an electromagnetic door latch supported by an electronic door lock with security function to prevent interruption to long exposure. There is high resolution cooled camera for noise free images with 5.5 effective mega pixels. Neutral field screens to automatically eliminate uneven light illumination and lens distortion effects. Captures and analyzes images stained with Ethidium Bromide, Silver stain and Comassive Blue.

5.

Thermo Cycler
Bangalore Genei
Equipped with a 20 vial Interchangeable Sample Blocks in this instrument. It shows a complete digital display profile and cycle sequencing with a heated lip facility. The most efficient heating and cooling rates (4-5C) are maintained. It supports a temperature range of 4-99C. There's a power input of 220-240VAC, 50Hz and also has auto restart facility.

6.

-80C Deep Freezer
Premium Upright
Model: U410
The freezer shows programmable controls. The interior stainless steel shelves can be easily cleaned. There are four interior compartment doors and five door option. The temperature ranges around -50C to -86C. The capacity is 13 cu ft or 368.1 liters with a RTD control monitor probe that works with the controller to maintain stable freezer temperature.

7.

-20C Deep Freezer
Carrier Model HF300
We have a -20C deep freezer for regular storage of protein samples for proteomics and molecular biology work. There are no compartments found apart from a small shelve. The temperature ranges from -4C to -20C

8.

Refrigerated Centrifuge 5415
Eppendorf
Table top centrifuge with a total volume of 500ml. It serves a temperature range of -9C to 40C. The fixed angle and swing out rotor helps in accommodating both bottom tubes as well as falcon tubes. The swing out rotor also accommodates 36 of 15ml falcon tubes or 48 of 15ml round bottom. Short spin with selectable rotational speed is one its features. Separate rotors for 1.5 - 2.0ml eppendorf tubes are found. A maximum of 12,000rpm is the rotation capacity.

9.

Cooling Centrifuge
Revolutionary table top REMI Model: C24BL)
The REMI cooling centrifuge has a maximum speed of 6000 RPM at 10 tube of 15 ml total square body, with lid lock and speed control by analogue. It also has a digital display of timer and the timer time has 0-60 minutes speed in digital display. Auto off lid open along with lid breaking system is provided. The power consumption is 500 watt and the requirements include 220V and 50 to 60 Hz.

10.

Bio-safety cabinet
BIOBASE BIOTECH
It belongs to class II type biological safety cabinet and used to provide protection to personnel, product and environment. It consists of advanced HEPA filter with the 99.99% filtration efficiency, vacuum fluorescent display which shows the work statues of equipment and it has the remote controller system to operate.

11.

Bench Top Freeze Dryer
ILSHIN - TFD5505 Table top model
Used for lyophilization purpose and stabilize the product, minimizing the effects of oxidation and other degradation processes, table top model has standard drum with 8 valves (1/2 inch) which is ideal for Connecting freeze dry flasks and ice capacity of about 3 liter / 24 hour with autorun programming facility.

12.

Water purification system
PURELAB OPTION-Q 7/15
It is ideal for a wide range of applications including molecular biology, bacterial and mammalian cell culture, electrophoresis, HPLC, ICP-MS, ICP-ES, and Ion Chromatography and it provides water free from Endotoxins, RNase and DNase .Consistent water quality is guaranteed at point of use through recirculation of water through integrated purification technologies and reservoir and Produces type I water directly from tap water

13.

Cold cabinet
UNICHROMAT 1500-PRO
Equipped with Interior and front panel made of stainless steel White housing, powder coated Digital temperature control with visual alarm Digital display in 0.5° C Potential-free outlet for connection to a house alarm system . Working temperature: +4° C constant . with amplified cooling performance, for compensating the heating emission of the Akta-System Regulation from +1° C to +15° C 2x PT-1000 temperature control sensor

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December 2017